Purity Testing & Quality Assurance
Every Bastion Peptides compound is independently verified at ≥99% purity before it ships. Here is what that means, how we test, and how to verify your batch.
HPLC Verification: What ≥99% Means
High-performance liquid chromatography (HPLC) separates a compound’s components by how they interact with a solvent and a stationary phase. The resulting chromatogram shows each component as a peak; the area under each peak represents its proportion of the total sample.
When we say ≥99% purity, we mean the target peptide accounts for ≥99% of the peak area in the chromatogram. The remaining ≤1% may include synthesis by-products, truncated sequences, or residual solvents — all at levels that do not affect research validity.
LC-MS Confirmation: Identity Verification
HPLC purity alone confirms proportion — it does not confirm identity. Liquid chromatography–mass spectrometry (LC-MS) adds a second verification layer: the instrument measures the mass-to-charge ratios of molecular fragments, producing a fingerprint that is compared against the theoretical molecular weight of the target compound.
Every Bastion Peptides batch undergoes both HPLC and LC-MS analysis. Both results appear on the Certificate of Analysis.
Third-Party Testing: Janoshik Analytical
Bastion Peptides uses Janoshik Analytical as its independent third-party testing laboratory. Janoshik is a Czech Republic–based analytical chemistry lab specialising in peptide and research chemical analysis, used by researchers and suppliers worldwide.
Independent testing means the lab has no commercial incentive to pass a sample — their obligation is accuracy. Batch samples are shipped directly to Janoshik; results are returned in the COA format you see on our Resources page.
How to Read a Certificate of Analysis
Each Janoshik Analytical COA contains the following fields:
| Field | What it means |
|---|---|
| Sample Name | The compound tested (e.g., BPC-157) |
| Batch / Lot Number | Matches the batch number printed on your vial label |
| HPLC Purity (%) | Percentage of target compound by chromatogram peak area — should read ≥99.0 |
| Observed MW | Measured molecular weight from LC-MS — should match theoretical MW ± 1 Da |
| Theoretical MW | Expected molecular weight of the compound at 100% purity |
| Test Date | Date the analysis was performed at Janoshik lab |
| Analyst Signature | Certifies the report was reviewed by a qualified analyst |
If your COA shows HPLC purity below 99.0% or an observed MW that deviates by more than 1 Da from theoretical, contact support@bastionpeptides.com — we will replace the batch.
Look Up Your Batch COA
The batch number is printed on your vial label. Enter it in the COA Lookup on the Resources page to access your Janoshik report.
COA Lookup → ResourcesFrequently Asked Questions
Does every batch get tested, or just a representative sample?
Every production batch is tested individually. We do not test a representative sample and extrapolate — each distinct synthesis batch receives its own Janoshik COA with a unique batch number.
Can I request a re-test of my batch?
Yes. If you have reason to question a COA result, contact support@bastionpeptides.com with your order number and batch number. We will arrange an independent re-test at no cost to you.
What is the difference between HPLC purity and peptide content?
HPLC purity (%) measures the proportion of the target compound relative to everything else in the sample. Peptide content (mg) is the absolute quantity in the vial. Bastion Peptides reports HPLC purity on all COAs; vial fill weights are confirmed by mass before dispatch.
Why is 99% purity the threshold and not 95% or 100%?
99% is the recognised research-grade threshold for peptides used in in vitro and in vivo studies. Values below 95% introduce impurities at levels that can measurably affect receptor-binding assays and dose-response curves. 100% is analytically unverifiable — chromatographic methods always operate within a detection threshold. 99%+ is the accepted industry standard for credible research-grade supply.
